Antimicrobial activity and toxicity of methanol extract of Cassia fistula seeds

The methanol extract of Cassia fistula seeds was investigated for potential antimicrobial activity against medically important bacterial, yeast and fungal strains. The antimicrobial activity was determined in methanol extract using the disk diffusion technique and the broth dilution method. The extract was effective on tested microorganism and the minimum inhibitory concentration (MIC) values were found in the range of 1.56350.00 mg/ml. Apart from that, the methanolic extract of C. fistula seeds was further tested for in vivo brine shrimp lethality test. The brine shrimp lethality test exhibited no significant toxicity (LC50 = 2.11mg/ml) against Artemia salina. The C. fistula seeds extract with high LC50 value signified that this plant is not toxic to human. Hence, this plant can be used to discover bioactive natural products that may leads in the development of new pharmaceuticals that address not fulfilled therapeutic needs.